She had to take a step back from her hosting duties this weekend as she self-isolates at home.
It seems Phillip Schofield will present the show by himself as Willoughby tested positive just hours before the semi final begins.
However, the This Morning star will return to the ice rink for her hosting duties for the Dancing on Ice Live Final.
A Dancing On Ice spokesperson said in a statement: “Unfortunately, Holly Willoughby is unable to host Sunday’s show after testing positive for Covid-19. We look forward to Holly returning for the Dancing on Ice Live Final.”
Schofield was forced to miss an earlier episode of Dancing on Ice when he was struck down with Covid.
At the time, Stephen Mulhern stepped up as a replacement.
Willoughby will miss this week’s This Morning and a rotation of celebs are expected to fill in for her while she is off.
It comes after Willoughby revealed her dog had eaten her hoover.
She wrote, “Dyson update… my dog ate my hoover…”